From Synbio 1.0 to Synbio 2.0

题  目: From Synbio 1.0 to Synbio 2.0

讲座人: 元英进教授

       Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University

时  间: 2月16日（周二）14:30-16:00

地  点: 卢嘉锡202报告厅

报告摘要：Standardization of parts and modules is one of the main milestones in the development of synthetic biology. By now, the standardization is coming to synbio2.0 from synbio1.0. in synbio1.0, the standardization meant the same sticky ends of DNA segments and the same endonucleases, However, the compatibility of different standardization was poor due to the different endonucleases for different libraries of parts. In synbio2.0, type IIS endonucleases are now introduced into the standardization of parts and modules. Type IIS endonucleases can generate the flexible ends to make most libraries compatible. In addition, the use of the type IIS endonuclease collection with the different binding sequences can avoid the pre-mutation of the binding sequences in the parts. Based on type IIS endonucleases, we have been developing a standard library (SynbioML@TJU) of synthetic parts and modules, which are optimized for codon biases of model microbes. More than 6,000 parts and modules have been deposited according to the metabolic pathways and products in SynbioML@TJU, and all of them are available for uncommercial academic researches. The website of SynbioML@TJU is
http://synbioml.org.